Some Windows 10 updates completely changed the USB stack. Your BIOS may be handling USB 3.0 in "XHCI" mode which breaks Setool2's timing.
Save and restart the software.
To prevent Windows 10 from breaking your Setool2 configuration during future system updates, consider implementing these practices: Setool2 Smart Card Not Found Windows 10 -
Expand the section at the bottom.
You need to kill the Windows Smart Card service (Fix #2). Some Windows 10 updates completely changed the USB stack